Title: Carbon catabolite repression of invertase during batch cultivations of Saccharomyces cerevisiae: the role of glucose, fructose, and mannose

標(biāo)題：葡萄糖、果糖與甘露糖對釀酒酵母菌批培養(yǎng)中轉(zhuǎn)化酶的碳分解產(chǎn)物抑制作用

Content/內(nèi)容：

1) When Saccharomyces cerevisiae are grown on a mixture of glucose and another fermentable sugar such as sucrose, maltose or galactose, the metabolism is diauxic, i. e. glucose is metabolized first, whereas the other sugars are metabolized when glucose is exhausted.

釀酒酵母菌生長在葡萄糖與其他發(fā)酵糖類（如蔗糖、麥芽糖、半乳糖）混合物中，代謝呈雙峰生長即首先是葡萄糖代謝；其次當(dāng)葡萄糖耗竭時(shí)，其他糖類才開始代謝。

2) This phenomenon is a consequence of glucose геpression, or more generally, catabolite repression.

此現(xiàn)象是葡萄糖受抑制，或更多的是分解產(chǎn)物受抑制的結(jié)果。

3) Besides glucose, the hexoses fructose and mannose are generally also believed to trigger catabolite repression.

除了葡萄糖以外，一般還認(rèn)為已糖類、果糖與甘露糖激發(fā)代謝抑制。

4) In this study, batch fermentations of S. cerevisiae in mixtures of sucrose and either glucose, fructose or mannose were performed.

在此研究中，進(jìn)行的是在蔗糖與葡萄糖、果糖或甘解糖混合物中釀酒酵母菌的批發(fā)酵。

5) It was found that the utilization of sucrose is inhibited by concentrations of either glucose or fructose higher than 5 g/L, and thus that glucose and fructose are equally capable of exerting catabolite repression.

發(fā)現(xiàn)葡萄糖或果糖高于5g/L，抑制蔗糖利用，這樣葡萄糖與果糖一樣能發(fā)揮抑制分解產(chǎn)物的作用。

6) However, sucrose was found to be hydrolyzed to glucose and fructose, even when the mannose concentration was as high as 17g/L, indicating that mannose is not a repressing sugar.

然而，蔗糖水解為葡萄糖與果糖；甚至當(dāng)甘露糖濃度高達(dá)17 g/L 時(shí)，也是如此，表明甘露糖無抑制作用。

7) It is suggested that the capability to trigger catabolite repression is connected to hexokinase P II, which is involved in the in vivo phosphorylation of glucose and fructose.

此提示激發(fā)分解產(chǎn)物受抑制作用與己糖磷酸激酶P II有關(guān)，而后者與體內(nèi)葡萄糖與果糖的磷酸化有關(guān)。

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